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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRAS Polyclonal antibody

MRAS Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 14213-1-AP

Synonyms

Ras related protein R Ras3, Ras related protein M Ras, R RAS3, muscle RAS oncogene homolog, M RAs

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRAS Polyclonal antibody (14213-1-AP)

    WB analysis using 14213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14213-1-AP (MRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using MRAS Polyclonal antibody (14213-1-AP)

    WB analysis of mouse lung using 14213-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14213-1-AP (MRAS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MRAS Polyclonal antibody (14213-1-AP)

    WB analysis of human brain using 14213-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14213-1-AP (MRAS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MRAS Polyclonal antibody (14213-1-AP)

    IP experiment of mouse brain using 14213-1-AP

    IP result of anti-MRAS (IP:14213-1-AP, 4ug; Detection:14213-1-AP 1:800) with mouse brain tissue lysate 2400 ug.

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Tested Applications

Positive WB detected inmouse brain tissue, mouse lung tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14213-1-AP targets MRAS in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRAS fusion protein Ag5433 相同性解析による交差性が予測される生物種
Full Name muscle RAS oncogene homolog
Calculated molecular weight 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC047690
Gene symbol MRAS
Gene ID (NCBI) 22808
RRIDAB_10950895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRAS antibody 14213-1-APDownload protocol
IP protocol for MRAS antibody 14213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep

Mutation-induced LZTR1 polymerization provokes cardiac pathology in recessive Noonan syndrome

Authors - Alexandra Viktoria Busley
View Article
humanWB

Stem Cell Res

Generation of a genetically-modified induced pluripotent stem cell line harboring a Noonan syndrome-associated gene variant MRAS p.G23V

Authors - Alexandra Viktoria Busley
View Article