Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
11639-1-AP targets MRFAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRFAP1 fusion protein Ag2227 相同性解析による交差性が予測される生物種 |
| Full Name | Mof4 family associated protein 1 |
| Calculated molecular weight | 127 aa, 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC022797 |
| Gene Symbol | MRFAP1 |
| Gene ID (NCBI) | 93621 |
| RRID | AB_2145271 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y605 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRFAP1, also named as PAM14 and PGR1, is a 14 kDa protein which associated with MRG. It is found in a complex composed of MORF4L1, MRFAP1 and RB1. MRFAP1 interacts via its N-terminus with MORF4L1. And it interacts with CSTB and MORF4L2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
| IHC protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
| IP protocol for MRFAP1 antibody 11639-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Characterisation of Native Protein Complexes and Protein Isoform Variation using Size-Fractionation Based Quantitative Proteomics. | ||
Mol Cell Proteomics Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. | ||
Oncotarget FBXW8-dependent degradation of MRFAP1 in anaphase controls mitotic cell death. |