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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRI1 Polyclonal antibody, PBS Only

MRI1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19916-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HT-1080 using 19916-1-AP (same clone as 19916-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using MRI1 Polyclonal antibody (19916-1-AP)

    WB analysis of HL-60 using 19916-1-AP (same clone as 19916-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19916-1-AP (MRI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using MRI1 Polyclonal antibody (19916-1-AP)

    IHC staining of rat kidney using 19916-1-AP (same clone as 19916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19916-1-AP (MRI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19916-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19916-1-PBS targets MRI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRI1 fusion protein Ag13809 相同性解析による交差性が予測される生物種
Full Name methylthioribose-1-phosphate isomerase homolog (S. cerevisiae)
Calculated molecular weight 369 aa, 39 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC001703
Gene symbol MRI1
Gene ID (NCBI) 84245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MRI1 is also named as MRDI and belongs to the eIF-2B alpha/beta/delta subunits family. MRI1 promotes cell invasion in response to constitutive RhoA activation by promoting FAK tyrosine phosphorylation (PMID:19620624). MRDI acts as a methylthioribose-1-phosphate (MTR-1-P) isomerase, catalyzing a critical step in methionine salvage. Second, MRDI promotes and is necessary for melanoma cell invasion, independent of its catalytic activity (PMID:23859498).