Validation Data Gallery
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Tested Applications
| Positive WB detected in | 37°C incubated HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
27825-1-AP targets MRP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRP1 fusion protein Ag27048 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-binding cassette, sub-family C (CFTR/MRP), member 1 |
| Calculated molecular weight | 172 kDa |
| Observed molecular weight | 180-190 kDa |
| GenBank accession number | NM_004996 |
| Gene Symbol | MRP1 |
| Gene ID (NCBI) | 4363 |
| RRID | AB_3085997 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Multidrug resistant-associated protein 1 (MRP1, also known as ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRP1 antibody 27825-1-AP | Download protocol |
| IHC protocol for MRP1 antibody 27825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol EYA4 reduces chemosensitivity of osteosarcoma to doxorubicin through DNA damage repair | ||
Drug Resist Updat RGS5+ lymphatic endothelial cells facilitate metastasis and acquired drug resistance of breast cancer through oxidative stress-sensing mechanism |