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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRP1 Monoclonal antibody (67228-1-Ig)

    WB analysis using 67228-1-Ig (same clone as 67228-1-PBS)

    Non-boil and boiled HepG2 and A549 cell lysates were subjected to SDS PAGE followed by western blot with 67228-1-Ig (ABCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Left panel was exposed for 20 sec. and right panel was exposed for 1 min. This data was developed using the same antibody clone with 67228-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MRP1 Monoclonal antibody (67228-1-Ig)

    IF Staining of A549 using 67228-1-Ig (same clone as 67228-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MRP1 antibody (67228-1-Ig, Clone: 1G4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67228-1-PBS in a different storage buffer formulation.

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MRP1 Monoclonal antibody, PBS Only

MRP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67228-1-PBS
Clone No.1G4A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ABCC1, MRP1/ABCC1, 1G4A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67228-1-PBS targets MRP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MRP1 fusion protein Ag27048 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Calculated molecular weight 172 kDa
Observed molecular weight 180-190 kDa
GenBank accession numberNM_004996
Gene Symbol MRP1
Gene ID (NCBI) 4363
RRIDAB_2882516
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33527
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Multidrug resistant-associated protein 1 (MRP1; ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.