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MRP63 Polyclonal antibody

MRP63 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21617-1-AP

Synonyms

MRPL57, Mitochondrial ribosomal protein L57, Large ribosomal subunit protein mL63, hMRP63, bMRP63

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MRP63 Polyclonal antibody (21617-1-AP)

    WB analysis of HeLa using 21617-1-AP

    WB result of MRP63 antibody (21617-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRP63 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using MRP63 Polyclonal antibody (21617-1-AP)

    WB analysis using 21617-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21617-1-AP (MRP63 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using MRP63 Polyclonal antibody (21617-1-AP)

    IHC staining of human colon using 21617-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21617-1-AP (MRP63 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MRP63 Polyclonal antibody (21617-1-AP)

    IHC staining of human colon using 21617-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21617-1-AP (MRP63 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21617-1-AP targets MRP63 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRP63 fusion protein Ag7289 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein 63
Calculated molecular weight 12 kDa
Observed molecular weight45 kDa
GenBank accession numberBC000002
Gene symbol MRP63
Gene ID (NCBI) 78988
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial ribosomal protein 63 is a component of the mitochondrial Large ribosomal subunit (mt-LSU), also known as the Large ribosomal subunit protein mL63.

Protocols

Product Specific Protocols
WB protocol for MRP63 antibody 21617-1-APDownload protocol
IHC protocol for MRP63 antibody 21617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols