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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL1 Polyclonal antibody

MRPL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16254-1-AP

Synonyms

MRP-L1, MRP L1, Large ribosomal subunit protein uL1m, L1mt, BM-022

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL1 Polyclonal antibody (16254-1-AP)

    WB analysis using 16254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16254-1-AP (MRPL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPL1 Polyclonal antibody (16254-1-AP)

    IHC staining of human lung cancer using 16254-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16254-1-AP (MRPL1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16254-1-AP targets MRPL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL1 fusion protein Ag9270 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L1
Calculated molecular weight 303 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC015109
Gene symbol MRPL1
Gene ID (NCBI) 65008
RRIDAB_2145582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL1 antibody 16254-1-APDownload protocol
IHC protocol for MRPL1 antibody 16254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
View Article