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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL10 Monoclonal antibody

MRPL10 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1C4

Cat no : 68623-1-Ig

Synonyms

L10MT, L8mt, MRP L10, MRP L8, MRPL10, MRPL8, RPML8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL10 Monoclonal antibody (68623-1-Ig)

    WB analysis using 68623-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68623-1-Ig (MRPL10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL10 Monoclonal antibody (68623-1-Ig)

    IF Staining of HeLa using 68623-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPL10 antibody (68623-1-Ig, Clone: 2A1C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, U2OS cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68623-1-Ig targets MRPL10 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MRPL10 fusion protein Ag10060 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L10
Calculated molecular weight 261 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC052601
Gene symbol MRPL10
Gene ID (NCBI) 124995
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL10 antibody 68623-1-IgDownload protocol
IF protocol for MRPL10 antibody 68623-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols