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  • Featured Product
  • KD/KO Validated

MRPL12 Polyclonal antibody

MRPL12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14795-1-AP

Synonyms

RPML12, MRPL7/L12, MRPL7, MRP-L12, Large ribosomal subunit protein bL12m

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL12 Polyclonal antibody (14795-1-AP)

    WB analysis using 14795-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14795-1-AP (MRPL12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MRPL12 Polyclonal antibody (14795-1-AP)

    WB analysis of HeLa using 14795-1-AP

    WB result of MRPL12 antibody (14795-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL12 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using MRPL12 Polyclonal antibody (14795-1-AP)

    IP experiment of HeLa using 14795-1-AP

    IP result of anti-MRPL12 (IP:14795-1-AP, 4ug; Detection:14795-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MRPL12 Polyclonal antibody (14795-1-AP)

    IHC staining of human colon cancer using 14795-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MRPL12 Polyclonal antibody (14795-1-AP)

    IHC staining of human colon cancer using 14795-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL12 Polyclonal antibody (14795-1-AP)

    IF Staining of HepG2 using 14795-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL12 Polyclonal antibody (14795-1-AP)

    IF Staining of HepG2 using 14795-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL12 Polyclonal antibody (14795-1-AP)

    IF Staining of HepG2 using 14795-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using MRPL12 Polyclonal antibody (14795-1-AP)

    FC experiment of HeLa using 14795-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug MRPL12 Polyclonal antibody (14795-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14795-1-AP targets MRPL12 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL12 fusion protein Ag6504 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L12
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC002344
Gene symbol MRPL12
Gene ID (NCBI) 6182
RRIDAB_2250805
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.

Protocols

Product Specific Protocols
WB protocol for MRPL12 antibody 14795-1-APDownload protocol
IHC protocol for MRPL12 antibody 14795-1-APDownload protocol
IF protocol for MRPL12 antibody 14795-1-APDownload protocol
IP protocol for MRPL12 antibody 14795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
View Article
WB

Mol Cell

A kinetic dichotomy between mitochondrial and nuclear gene expression processes

Authors - Erik McShane
View Article
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
View Article
humanWB

Nucleic Acids Res

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Authors - Lindsey Van Haute
View Article
humanWB

Genome Biol

Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes

Authors - Iliana Soto
View Article