Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 14795-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14795-1-AP (MRPL12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14795-1-AP
WB result of MRPL12 antibody (14795-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL12 transfected HeLa cells.
IP experiment of HeLa using 14795-1-AP
IP result of anti-MRPL12 (IP:14795-1-AP, 4ug; Detection:14795-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human colon cancer using 14795-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 14795-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14795-1-AP (MRPL12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14795-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 14795-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 14795-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (14795-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HeLa using 14795-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug MRPL12 Polyclonal antibody (14795-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

MRPL12 Polyclonal antibody

MRPL12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14795-1-AP

Publications(37)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7, MRPL7/L12

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 555 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (37)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 33 publications below
IHC	See 4 publications below
IF	See 7 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

14795-1-AP targets MRPL12 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6504 Product name: Recombinant human MRPL12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC002344 Sequence: MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE 相同性解析による交差性が予測される生物種
Full Name	mitochondrial ribosomal protein L12
Calculated molecular weight	21 kDa
Observed molecular weight	21 kDa
GenBank accession number	BC002344
Gene Symbol	MRPL12
Gene ID (NCBI)	6182
RRID	AB_2250805
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P52815
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression. The antibody reacts specifically with 21kDa MRPL12 protein.

Protocols

Product Specific Protocols
IF protocol for MRPL12 antibody 14795-1-AP	Download protocol
IHC protocol for MRPL12 antibody 14795-1-AP	Download protocol
IP protocol for MRPL12 antibody 14795-1-AP	Download protocol
WB protocol for MRPL12 antibody 14795-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell An in vitro system to silence mitochondrial gene expression. Authors - Luis Daniel Cruz-Zaragoza View Article
human	WB	Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria Authors - Sabine Poerschke View Article
	WB	Mol Cell A kinetic dichotomy between mitochondrial and nuclear gene expression processes Authors - Erik McShane View Article
human	WB	Nucleic Acids Res The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis. Authors - Elena Lavdovskaia View Article
		EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. Authors - Nicola Ferreira View Article
human	WB	Nucleic Acids Res METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. Authors - Lindsey Van Haute View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14795-1-AP

WB analysis of HeLa using 14795-1-AP

IP experiment of HeLa using 14795-1-AP

IHC staining of human colon cancer using 14795-1-AP

IHC staining of human colon cancer using 14795-1-AP

IF Staining of HepG2 using 14795-1-AP

IF Staining of HepG2 using 14795-1-AP

IF Staining of HepG2 using 14795-1-AP

FC experiment of HeLa using 14795-1-AP

MRPL12 Polyclonal antibody

MRPL12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An in vitro system to silence mitochondrial gene expression.

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

A kinetic dichotomy between mitochondrial and nuclear gene expression processes

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.