Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16241-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16241-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 16241-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16241-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16241-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 16241-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COS-7 using 16241-1-AP
COS-7 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 16241-1-AP
IP result of anti-MRPL13 (IP:16241-1-AP, 4ug; Detection:16241-1-AP 1:500) with mouse lung tissue lysate 4000ug.
IHC staining of human liver cancer using 16241-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 16241-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16241-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 16241-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL13 Polyclonal antibody

MRPL13 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16241-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, ELISA

Synonyms

L13, L13A, L13mt, MRP L13, MRPL13, RPL13, RPML13

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HL-60 cells, HeLa cells, mouse lung tissue, Jurkat cells, COS-7 cells, rat lung tissue
Positive IP detected in	mouse lung tissue
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 1 publications below

Product Information

16241-1-AP targets MRPL13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MRPL13 fusion protein Ag9105 相同性解析による交差性が予測される生物種
Full Name	mitochondrial ribosomal protein L13
Calculated molecular weight	178 aa, 21 kDa
Observed molecular weight	20-23 kDa
GenBank accession number	BC009190
Gene Symbol	MRPL13
Gene ID (NCBI)	28998
RRID	AB_2145705
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BYD1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MRPL13, also named as 39S ribosomal protein L13, mitochondrial or L13mt, is a 178 amino acid protein, which belongs to the ribosomal protein L13P family. MRPL13 localizes in the mitochondrion and interacts with OXA1L. MRPL13 is involved in mitochondrial proteins biosynthesis, ribosomal structure and biogenesis.

Protocols

Product Specific Protocols
WB protocol for MRPL13 antibody 16241-1-AP	Download protocol
IHC protocol for MRPL13 antibody 16241-1-AP	Download protocol
IP protocol for MRPL13 antibody 16241-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis. Authors - Elena Lavdovskaia View Article
human	WB	Nucleic Acids Res Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. Authors - Surovtseva Yulia V YV View Article
human	WB	Nucleic Acids Res MPV17L2 is required for ribosome assembly in mitochondria. Authors - Ilaria Dalla Rosa View Article
human	WB	Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. Authors - Ruona Shi View Article
human	WB	J Cell Sci CLUH couples mitochondrial distribution to the energetic and metabolic status. Authors - Jamal Wakim View Article
human	WB	Life (Basel) Molecular Investigation of Mitochondrial RNA19 Role in the Pathogenesis of MELAS Disease Authors - Paola Loguercio Polosa View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16241-1-AP

WB analysis of HeLa using 16241-1-AP

WB analysis of HL-60 using 16241-1-AP

WB analysis of mouse lung using 16241-1-AP

WB analysis of Jurkat using 16241-1-AP

WB analysis of rat lung using 16241-1-AP

WB analysis of COS-7 using 16241-1-AP

IP experiment of mouse lung using 16241-1-AP

IHC staining of human liver cancer using 16241-1-AP

IHC staining of human liver cancer using 16241-1-AP

IHC staining of human breast cancer using 16241-1-AP

IHC staining of human breast cancer using 16241-1-AP

MRPL13 Polyclonal antibody

MRPL13 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.

MPV17L2 is required for ribosome assembly in mitochondria.

Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.

CLUH couples mitochondrial distribution to the energetic and metabolic status.

Molecular Investigation of Mitochondrial RNA19 Role in the Pathogenesis of MELAS Disease