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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL16 Polyclonal antibody (14762-1-AP)

    WB analysis using 14762-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14762-1-AP (MRPL16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MRPL16 Polyclonal antibody (14762-1-AP)

    IP experiment of HeLa using 14762-1-AP

    IP result of anti-MRPL16 (IP:14762-1-AP, 4ug; Detection:14762-1-AP 1:500) with HeLa cells lysate 1520 ug.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using MRPL16 Polyclonal antibody (14762-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 14762-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14762-1-AP (MRPL16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MRPL16 Polyclonal antibody

MRPL16 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14762-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

PNAS-111, PNAS 111, MRP-L16, MRP L16, Large ribosomal subunit protein uL16m

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14762-1-AP targets MRPL16 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL16 fusion protein Ag6523 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L16
Calculated molecular weight 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC001040
Gene Symbol MRPL16
Gene ID (NCBI) 54948
RRIDAB_3085442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX20
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPL16 (Mitochondrial Ribosomal Protein L16) is a protein coding gene. It encodes a 39S subunit protein. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. The molecular mass of MRPL16 is 28 kDa.

Protocols

Product Specific Protocols
WB protocol for MRPL16 antibody 14762-1-APDownload protocol
IHC protocol for MRPL16 antibody 14762-1-APDownload protocol
IP protocol for MRPL16 antibody 14762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols