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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MRPL23 Monoclonal antibody (66595-1-Ig)

    WB analysis of HEK-293 using 66595-1-Ig (same clone as 66595-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66595-1-Ig (MRPL23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL23 Monoclonal antibody (66595-1-Ig)

    IHC staining of human breast cancer using 66595-1-Ig (same clone as 66595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66595-1-Ig (MRPL23 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL23 Monoclonal antibody (66595-1-Ig)

    IHC staining of human breast cancer using 66595-1-Ig (same clone as 66595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66595-1-Ig (MRPL23 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66595-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MRPL23 Monoclonal antibody (66595-1-Ig)

    IF Staining of HEK-293 using 66595-1-Ig (same clone as 66595-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66595-1-Ig (MRPL23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66595-1-PBS in a different storage buffer formulation.

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MRPL23 Monoclonal antibody, PBS Only

MRPL23 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66595-1-PBS
Clone No.1G11C4

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

L23MRP, L23mt, MRP L23, MRPL23, Ribosomal protein L23 like, RPL23, RPL23L

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66595-1-PBS targets MRPL23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MRPL23 fusion protein Ag2220 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L23
Calculated molecular weight 153 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC027710
Gene Symbol MRPL23
Gene ID (NCBI) 6150
RRIDAB_2881955
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16540
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.