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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL33 Polyclonal antibody

MRPL33 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23870-1-AP

Synonyms

C2orf1, L33mt, MRP L33, MRPL33, RPL33L

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL33 Polyclonal antibody (23870-1-AP)

    IHC staining of human kidney using 23870-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23870-1-AP (MRPL33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPL33 Polyclonal antibody (23870-1-AP)

    IHC staining of human kidney using 23870-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23870-1-AP (MRPL33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL33 Polyclonal antibody (23870-1-AP)

    IF Staining of HepG2 using 23870-1-AP

    Immunofluorescent analysis of HepG2 cells using 23870-1-AP (MRPL33 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23870-1-AP is MRPL33 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL33 fusion protein Ag20921 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L33
Calculated molecular weight 65 aa, 8 kDa
GenBank accession numberBC009475
Gene symbol MRPL33
Gene ID (NCBI) 9553
RRIDAB_2879345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPL33 is a mitochondrial ribosomal protein. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.

Protocols

Product Specific Protocols
IHC protocol for MRPL33 antibody 23870-1-APDownload protocol
IF protocol for MRPL33 antibody 23870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols