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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL34 Polyclonal antibody

MRPL34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15233-1-AP

Synonyms

MRP-L34, MRP L34, Large ribosomal subunit protein bL34m, L34mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL34 Polyclonal antibody (15233-1-AP)

    WB analysis using 15233-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15233-1-AP (MRPL34 antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using MRPL34 Polyclonal antibody (15233-1-AP)

    IHC staining of mouse lung using 15233-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15233-1-AP (MRPL34 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using MRPL34 Polyclonal antibody (15233-1-AP)

    IHC staining of mouse lung using 15233-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15233-1-AP (MRPL34 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL34 Polyclonal antibody (15233-1-AP)

    IF Staining of HepG2 using 15233-1-AP

    Immunofluorescent analysis of HepG2 cells using 15233-1-AP (MRPL34 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15233-1-AP targets MRPL34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL34 fusion protein Ag7173 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L34
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC000071
Gene symbol MRPL34
Gene ID (NCBI) 64981
RRIDAB_2145915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL34 antibody 15233-1-APDownload protocol
IHC protocol for MRPL34 antibody 15233-1-APDownload protocol
IF protocol for MRPL34 antibody 15233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols