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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL37 Polyclonal antibody

MRPL37 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29522-1-AP

Synonyms

MRP L37, MRP L2, Large ribosomal subunit protein mL37, L37mt, L2mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL37 Polyclonal antibody (29522-1-AP)

    WB analysis using 29522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29522-1-AP (MRPL37 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using MRPL37 Polyclonal antibody (29522-1-AP)

    IHC staining of human stomach cancer using 29522-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29522-1-AP (MRPL37 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL37 Polyclonal antibody (29522-1-AP)

    IF Staining of HepG2 using 29522-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPL37 antibody (29522-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, U-251 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29522-1-AP targets MRPL37 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL37 fusion protein Ag31452 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L37
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC000041
Gene symbol MRPL37
Gene ID (NCBI) 51253
RRIDAB_2918321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL37 antibody 29522-1-APDownload protocol
IHC protocol for MRPL37 antibody 29522-1-APDownload protocol
IF protocol for MRPL37 antibody 29522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols