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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL38 Polyclonal antibody

MRPL38 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 15913-1-AP

Synonyms

MRP L38, MRP L3, Large ribosomal subunit protein mL38, L38mt, KIAA1863

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL38 Polyclonal antibody (15913-1-AP)

    WB analysis using 15913-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15913-1-AP (MRPL38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using MRPL38 Polyclonal antibody (15913-1-AP)

    IHC staining of human liver using 15913-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MRPL38 Polyclonal antibody (15913-1-AP)

    IHC staining of human liver using 15913-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MRPL38 Polyclonal antibody (15913-1-AP)

    IHC staining of mouse brain using 15913-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MRPL38 Polyclonal antibody (15913-1-AP)

    IHC staining of mouse brain using 15913-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293 cells using MRPL38 Polyclonal antibody (15913-1-AP)

    FC experiment of HEK-293 using 15913-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug MRPL38 Polyclonal antibody (15913-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15913-1-AP targets MRPL38 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL38 fusion protein Ag8706 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L38
Calculated molecular weight 346 aa, 41 kDa
Observed molecular weight37-45 kDa
GenBank accession numberBC013311
Gene symbol MRPL38
Gene ID (NCBI) 64978
RRIDAB_2146047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL38 antibody 15913-1-APDownload protocol
IHC protocol for MRPL38 antibody 15913-1-APDownload protocol
FC protocol for MRPL38 antibody 15913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling

Authors - Jana Key
View Article
ratWB

Cell Rep

Small RNAs derived from tRNA fragmentation regulate the functional maturation of neonatal β cells.

Authors - Mustafa Bilal Bayazit
View Article
IHC

J Toxicol Sci

Aberrant epigenetic gene regulation in hippocampal neurogenesis of mouse offspring following maternal exposure to 3,3'-iminodipropionitrile.

Authors - Takeshi Tanaka
View Article