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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL40 Polyclonal antibody

MRPL40 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16767-1-AP

Synonyms

NLVCF, MRP-L40, MRP L40, MRP L22, Large ribosomal subunit protein mL40

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL40 Polyclonal antibody (16767-1-AP)

    WB analysis using 16767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16767-1-AP (MRPL40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using MRPL40 Polyclonal antibody (16767-1-AP)

    IP experiment of HeLa using 16767-1-AP

    IP result of anti-MRPL40 (IP:16767-1-AP, 4ug; Detection:16767-1-AP 1:2000) with HeLa cells lysate 1920 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using MRPL40 Polyclonal antibody (16767-1-AP)

    IHC staining of human ovary cancer using 16767-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16767-1-AP (MRPL40 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16767-1-AP targets MRPL40 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL40 fusion protein Ag10341 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L40
Calculated molecular weight 206 aa, 24 kDa
Observed molecular weight18-24 kDa
GenBank accession numberBC009707
Gene symbol MRPL40
Gene ID (NCBI) 64976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL40 antibody 16767-1-APDownload protocol
IHC protocol for MRPL40 antibody 16767-1-APDownload protocol
IP protocol for MRPL40 antibody 16767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols