Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, PC-3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17300-1-AP targets MRPL42 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRPL42 fusion protein Ag11181 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L42 |
| Calculated molecular weight | 142 aa, 17 kDa |
| Observed molecular weight | 14-17 kDa |
| GenBank accession number | BC040240 |
| Gene Symbol | MRPL42 |
| Gene ID (NCBI) | 28977 |
| RRID | AB_2935442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6G3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL42 antibody 17300-1-AP | Download protocol |
| IHC protocol for MRPL42 antibody 17300-1-AP | Download protocol |
| IF protocol for MRPL42 antibody 17300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |