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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL43 Polyclonal antibody

MRPL43 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17477-1-AP

Synonyms

bMRP36a, L43mt, MRP L43, MRPL43

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL43 Polyclonal antibody (17477-1-AP)

    WB analysis using 17477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17477-1-AP (MRPL43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MRPL43 Polyclonal antibody (17477-1-AP)

    IHC staining of mouse skeletal muscle using 17477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17477-1-AP (MRPL43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MRPL43 Polyclonal antibody (17477-1-AP)

    IHC staining of mouse skeletal muscle using 17477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17477-1-AP (MRPL43 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17477-1-AP is MRPL43 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL43 fusion protein Ag11498 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L43
Calculated molecular weight159aa,18 kDa; 215aa,23 kDa
Observed molecular weight17-23 kDa
GenBank accession numberBC015905
Gene symbol MRPL43
Gene ID (NCBI) 84545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPL43 antibody 17477-1-APDownload protocol
IHC protocol for MRPL43 antibody 17477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols