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  • Featured Product
  • KD/KO Validated

MRPL45 Monoclonal antibody, PBS Only

MRPL45 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4B4

Cat no : 66531-1-PBS

Synonyms

L45mt, MRPL45, MRP-L45

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

    WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis of HSC-T6 using 66531-1-Ig (same clone as 66531-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using MRPL45 Monoclonal antibody (66531-1-Ig)

    WB analysis of RAW 264.7 using 66531-1-Ig (same clone as 66531-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL45 Monoclonal antibody (66531-1-Ig)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPL45 Monoclonal antibody (66531-1-Ig)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66531-1-PBS targets MRPL45 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MRPL45 fusion protein Ag8207 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L45
Calculated molecular weight 35 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC006235
Gene symbol MRPL45
Gene ID (NCBI) 84311
RRIDAB_2881894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.