Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse brain tissue, mouse heart tissue |
| Positive IHC detected in | human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
16611-1-AP targets MRPL46 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRPL46 fusion protein Ag9837 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L46 |
| Calculated molecular weight | 279 aa, 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC017883 |
| Gene Symbol | MRPL46 |
| Gene ID (NCBI) | 26589 |
| RRID | AB_2146074 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2W6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL46 antibody 16611-1-AP | Download protocol |
| IHC protocol for MRPL46 antibody 16611-1-AP | Download protocol |
| IF protocol for MRPL46 antibody 16611-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation | ||
iScience Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation | ||
EMBO J Non-canonical phosphoglycerate dehydrogenase activity promotes liver cancer growth via mitochondrial translation and respiratory metabolism |