Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IF | See 1 publications below |
Product Information
14677-1-AP targets MRPL48 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRPL48 fusion protein Ag6387 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein L48 |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 20-21 kDa |
| GenBank accession number | BC009764 |
| Gene Symbol | MRPL48 |
| Gene ID (NCBI) | 51642 |
| RRID | AB_2282151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96GC5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MRPL48, also named as CGI-118, or HSPC290 is a 212 amino acid protein, which localizes in the Mitochondrion. MRPL48 is a component of the large subunit of the mitochondrial ribosome, including two RNA molecules and, at least, 70 proteins among which 47 proteins for the large subunit. mitochondrial ribosomal protein helps in protein synthesis within the mitochondrion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL48 antibody 14677-1-AP | Download protocol |
| IHC protocol for MRPL48 antibody 14677-1-AP | Download protocol |
| IF protocol for MRPL48 antibody 14677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function. | ||
Nucleic Acids Res Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. | ||
Int J Mol Sci Low Expression of Mitofusin 1 Gene Leads to Mitochondrial Dysfunction and Embryonic Genome Activation Failure in Ovine-Bovine Inter-Species Cloned Embryos | ||
Cell Rep MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. | ||
Biomedicines Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema |