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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL51 Recombinant antibody (83565-1-RR)

    WB analysis using 83565-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83565-1-RR (MRPL51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MRPL51 Recombinant antibody (83565-1-RR)

    WB analysis of HEK-293 using 83565-1-RR

    WB result of MRPL51 antibody (83565-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL51 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using MRPL51 Recombinant antibody (83565-1-RR)

    IHC staining of human ovarian cancer using 83565-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPL51 Recombinant antibody (83565-1-RR)

    IHC staining of human lung cancer using 83565-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPL51 Recombinant antibody (83565-1-RR)

    IHC staining of human lung cancer using 83565-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using MRPL51 Recombinant antibody (83565-1-RR)

    IF Staining of HEK-293 using 83565-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MRPL51 antibody (83565-1-RR, Clone: 240381C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83565-1-RR

    Affinity and Kinetic Characterization of 83565-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83565-1-RR against Human MRPL51 were performed. The affinity constant is 0.57 nM.

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  • Featured Product
  • KD/KO Validated

MRPL51 Recombinant antibody

MRPL51 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83565-1-RR
Clone No.240381C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HSPC241, CDA09, bMRP-64, bMRP64, bMRP 64

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHUVEC cells, HEK-293 cells, SKOV-3 cells
Positive IHC detected inhuman lung cancer tissue, human ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83565-1-RR targets MRPL51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MRPL51 fusion protein Ag7193 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L51
Calculated molecular weight 15 kDa
Observed molecular weight12 kDa
GenBank accession numberBC000191
Gene Symbol MRPL51
Gene ID (NCBI) 51258
RRIDAB_3671180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ4U2R6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial ribosome protein L51 (MRPL51) is a 39S subunit protein of the mitochondrial ribosome. MRPL51 functions in the translation of genetic messages (PMID: 21730110). MRPL51 expression was positively correlated with cell cycle, DNA damage, DNA repair, epithelial-mesenchymal transition, invasion, and proliferation of LUAD cells at the singlecell level (PMID: 37323822). The calculated molecular weight of MRPL51 is 15 kDa.

Protocols

Product Specific Protocols
WB protocol for MRPL51 antibody 83565-1-RRDownload protocol
IHC protocol for MRPL51 antibody 83565-1-RRDownload protocol
IF protocol for MRPL51 antibody 83565-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols