MRPL55 Polyclonal antibody

MRPL55 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17679-1-AP

Synonyms

MRP L55, Mitochondrial large ribosomal subunit protein bL31m, Large ribosomal subunit protein mL55, L55nt, L55mt


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Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17679-1-AP targets MRPL55 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL55 fusion protein Ag11716 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L55
Calculated molecular weight 128 aa, 15 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC052806
Gene symbol MRPL55
Gene ID (NCBI) 128308
RRIDAB_2878427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPL55, also named as L55mt and MRP-L55, is a component of the mitochondrial ribosome large subunit (39S). It acts dynamically in the cell during development. The MW of MRPL55 is about 13 kDa.

Protocols

Product Specific Protocols
WB protocol for MRPL55 antibody 17679-1-APDownload protocol
IHC protocol for MRPL55 antibody 17679-1-APDownload protocol
IP protocol for MRPL55 antibody 17679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

Authors - Liang Wang
humanWB

Mol Biol Cell

Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.

Authors - Katarzyna Justyna Chojnacka
human,mouseWB

Cell Rep

OCIAD1 and prohibitins regulate the stability of the TIM23 protein translocase

Authors - Praveenraj Elancheliyan