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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPP3 Monoclonal antibody

MRPP3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G9A10

Cat no : 68168-1-Ig

Synonyms

KIAA0391, MRPP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPP3 Monoclonal antibody (68168-1-Ig)

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (MRPP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MRPP3 Monoclonal antibody (68168-1-Ig)

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MRPP3 Monoclonal antibody (68168-1-Ig)

    WB analysis using 68168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68168-1-Ig (KIAA0391 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, HEK-293 cells, K-562 cells, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68168-1-Ig targets MRPP3 in WB, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity Human, Mouse, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MRPP3 fusion protein Ag15629 相同性解析による交差性が予測される生物種
Full Name KIAA0391
Calculated molecular weight 583 aa, 67 kDa
Observed molecular weight 65-67 kDa
GenBank accession numberBC032221
Gene symbol MRPP3
Gene ID (NCBI) 9692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)

Protocols

Product Specific Protocols
WB protocol for MRPP3 antibody 68168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols