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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS11 Polyclonal antibody

MRPS11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17041-1-AP

Synonyms

HCC 2, MRP S11, MRPS11, RPMS11, S11mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS11 Polyclonal antibody (17041-1-AP)

    WB analysis using 17041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17041-1-AP (MRPS11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPS11 Polyclonal antibody (17041-1-AP)

    IHC staining of human kidney using 17041-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17041-1-AP (MRPS11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPS11 Polyclonal antibody (17041-1-AP)

    IHC staining of human kidney using 17041-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17041-1-AP (MRPS11 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17041-1-AP targets MRPS11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS11 fusion protein Ag10570 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S11
Calculated molecular weight 193 aa, 20 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC012489
Gene symbol MRPS11
Gene ID (NCBI) 64963
RRIDAB_2182495
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPS11 antibody 17041-1-APDownload protocol
IHC protocol for MRPS11 antibody 17041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.

Authors - Christopher Lowden
View Article