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MRPS16 Polyclonal antibody

Name: MRPS16 Polyclonal antibody
Brand: Proteintech
SKU: 16735-1-AP

MRPS16 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16735-1-AP

Synonyms

MRPS 16, MRP S16, COXPD2, CGI-132, CGI132

Validation Data Gallery

Filter:

AllWBIPIF/ICC

WB analysis using 16735-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16735-1-AP (MRPS16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16735-1-AP
WB result of MRPS16 antibody (16735-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS16 transfected HeLa cells.
WB analysis of HEK-293 using 16735-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16735-1-AP (MRPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16735-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16735-1-AP (MRPS16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 16735-1-AP
IP result of anti-MRPS16 (IP:16735-1-AP, 4ug; Detection:16735-1-AP 1:500) with HEK-293 cells lysate 1480ug.
IF Staining of HepG2 using 16735-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16735-1-AP (MRPS16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16735-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HEK-293 cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 18 publications below

Product Information

16735-1-AP targets MRPS16 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MRPS16 fusion protein Ag9749 相同性解析による交差性が予測される生物種
Full Name	mitochondrial ribosomal protein S16
Calculated molecular weight	137 aa, 15 kDa
Observed molecular weight	15 kDa
GenBank accession number	BC021106
Gene symbol	MRPS16
Gene ID (NCBI)	51021
RRID	AB_2180166
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPS16 antibody 16735-1-AP	Download protocol
IF protocol for MRPS16 antibody 16735-1-AP	Download protocol
IP protocol for MRPS16 antibody 16735-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Sci Adv Adult-onset obesity is triggered by impaired mitochondrial gene expression. Authors - Kara L Perks View Article
mouse	WB	Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. Authors - Danielle L Rudler View Article
human	WB	Nucleic Acids Res The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis. Authors - Elena Lavdovskaia View Article
mouse	WB	Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. Authors - Tara R Richman View Article
mouse	WB	EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. Authors - Nicola Ferreira View Article
mouse	WB	Aging Cell Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan. Authors - Tara R Richman View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS16 Polyclonal antibody

MRPS16 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 16735-1-AP

WB analysis of HeLa using 16735-1-AP

WB analysis of HEK-293 using 16735-1-AP

WB analysis of Jurkat using 16735-1-AP

IP experiment of HEK-293 using 16735-1-AP

IF Staining of HepG2 using 16735-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.