Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 18 publications below |
Product Information
16735-1-AP targets MRPS16 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MRPS16 fusion protein Ag9749 相同性解析による交差性が予測される生物種 |
| Full Name | mitochondrial ribosomal protein S16 |
| Calculated molecular weight | 137 aa, 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC021106 |
| Gene Symbol | MRPS16 |
| Gene ID (NCBI) | 51021 |
| RRID | AB_2180166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3D3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPS16 antibody 16735-1-AP | Download protocol |
| IF protocol for MRPS16 antibody 16735-1-AP | Download protocol |
| IP protocol for MRPS16 antibody 16735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. | ||
Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. | ||
EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. | ||
Aging Cell Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan. |