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  • Featured Product
  • KD/KO Validated

MRPS16 Recombinant antibody

MRPS16 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

11D12

Cat no : 82741-2-RR

Synonyms

MRP S16, COXPD2, CGI-132, CGI132, CGI 132

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MRPS16 Recombinant antibody (82741-2-RR)

    WB analysis of HeLa using 82741-2-RR

    WB result of MRPS16 antibody (82741-2-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS16 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using MRPS16 Recombinant antibody (82741-2-RR)

    WB analysis using 82741-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82741-2-RR (MRPS16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using MRPS16 Recombinant antibody (82741-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82741-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82741-2-RR (MRPS16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82741-2-RR

    Affinity and Kinetic Characterization of 82741-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82741-2-RR against Human MRPS16 were performed. The affinity constant is 0.616 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, HT-1080 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82741-2-RR targets MRPS16 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MRPS16 fusion protein Ag9749 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S16
Calculated molecular weight 137 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC021106
Gene symbol MRPS16
Gene ID (NCBI) 51021
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPS16 antibody 82741-2-RRDownload protocol
IHC protocol for MRPS16 antibody 82741-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols