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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS18A Polyclonal antibody

MRPS18A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16235-1-AP

Synonyms

MRP S18 a, MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS18A Polyclonal antibody (16235-1-AP)

    WB analysis using 16235-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16235-1-AP (MRPS18A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using MRPS18A Polyclonal antibody (16235-1-AP)

    IHC staining of human stomach using 16235-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16235-1-AP (MRPS18A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using MRPS18A Polyclonal antibody (16235-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 16235-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16235-1-AP (MRPS18A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16235-1-AP targets MRPS18A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS18A fusion protein Ag8981 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S18A
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight 18-22 kDa
GenBank accession numberBC010364
Gene symbol MRPS18A
Gene ID (NCBI) 55168
RRIDAB_2266663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPS18A (Mitochondrial Ribosomal Protein S18A) is a protein coding gene. It encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The increased expression of MRPS18A in cancer cells is explained by the increased energy metabolism of cancer cells and may pave the way for new diagnostic and therapeutic avenues to be explored (PMID: 28056857).

Protocols

Product Specific Protocols
WB protocol for MRPS18A antibody 16235-1-APDownload protocol
IHC protocol for MRPS18A antibody 16235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Exp Oncol

Expression pattern of MRPS18 family genes in malignantly transformed b-cells.

Authors - L Kovalevska
View Article