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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS18A Recombinant antibody (84201-1-RR)

    WB analysis using 84201-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84201-1-RR (MRPS18A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MRPS18A Recombinant antibody (84201-1-RR)

    WB analysis of HepG2 using 84201-1-RR

    WB result of MRPS18A antibody (84201-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS18A transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 84201-1-RR

    Affinity and Kinetic Characterization of 84201-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84201-1-RR against Human MRPS18A were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

MRPS18A Recombinant antibody

MRPS18A Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84201-1-RR
Clone No.240850C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial, 39S ribosomal protein S18-3, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, L02 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84201-1-RR targets MRPS18A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MRPS18A fusion protein Ag9011 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S18A
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight15 kDa
GenBank accession numberBC010364
Gene Symbol MRPS18A
Gene ID (NCBI) 55168
RRIDAB_3671757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NVS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPS18A (Mitochondrial Ribosomal Protein S18A) is a protein coding gene. It encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The increased expression of MRPS18A in cancer cells is explained by the increased energy metabolism of cancer cells and may pave the way for new diagnostic and therapeutic avenues to be explored (PMID: 28056857).

Protocols

Product Specific Protocols
WB protocol for MRPS18A antibody 84201-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols