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  • KD/KO Validated

MRPS25 Monoclonal antibody

MRPS25 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B10B6

Cat no : 67903-1-Ig

Synonyms

DKFZp313H0817, FLJ00023, MRP S25, MRPS25, RPMS25, S25mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS25 Monoclonal antibody (67903-1-Ig)

    WB analysis using 67903-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using MRPS25 Monoclonal antibody (67903-1-Ig)

    WB analysis using 67903-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67903-1-Ig (MRPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MRPS25 Monoclonal antibody (67903-1-Ig)

    WB analysis of HepG2 using 67903-1-Ig

    WB result of MRPS25 antibody (67903-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS25 transfected HepG2 cells.

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67903-1-Ig targets MRPS25 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MRPS25 fusion protein Ag7846 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S25
Calculated molecular weight 20 kDa
Observed molecular weight20 kDa
GenBank accession numberBC003590
Gene symbol MRPS25
Gene ID (NCBI) 64432
RRIDAB_2918659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitochondrial ribosomal protein S25 (MRPS25) belongs to the ribosomal protein S25/L51 family. It is a component of the mitochondrial ribosome small subunit (28S). The variant segregated with the disease and substitutes a highly conserved proline residue with leucine (p.P72L) that, based on the high-resolution structure of the 28S ribosome, is predicted to compromise inter-protein contacts and destabilize the small subunit.

Protocols

Product Specific Protocols
WB protocol for MRPS25 antibody 67903-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols