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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS26 Polyclonal antibody

MRPS26 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15989-1-AP

Synonyms

C20orf193, GI008, MRP S13, MRP S26, MRPS13, MRPS26, NY BR 87, RPMS13, S13mt, S26mt

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MRPS26 Polyclonal antibody (15989-1-AP)

    WB analysis of HepG2 using 15989-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MRPS26 Polyclonal antibody (15989-1-AP)

    WB analysis of HeLa using 15989-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MRPS26 Polyclonal antibody (15989-1-AP)

    WB analysis of mouse brain using 15989-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using MRPS26 Polyclonal antibody (15989-1-AP)

    IP experiment of HepG2 using 15989-1-AP

    IP result of anti-MRPS26 (IP:15989-1-AP, 4ug; Detection:15989-1-AP 1:1000) with HepG2 cells lysate 1085 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MRPS26 Polyclonal antibody (15989-1-AP)

    IHC staining of human liver cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MRPS26 Polyclonal antibody (15989-1-AP)

    IHC staining of human skin cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MRPS26 Polyclonal antibody (15989-1-AP)

    IHC staining of human liver cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MRPS26 Polyclonal antibody (15989-1-AP)

    IHC staining of human skin cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman skin cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15989-1-AP targets MRPS26 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS26 fusion protein Ag8797 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S26
Calculated molecular weight 205 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC013018
Gene symbol MRPS26
Gene ID (NCBI) 64949
RRIDAB_10858479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPS26 antibody 15989-1-APDownload protocol
IHC protocol for MRPS26 antibody 15989-1-APDownload protocol
IP protocol for MRPS26 antibody 15989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation

Authors - Taru Hilander
View Article
mouseWB

Life Sci Alliance

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Authors - Uwe Richter
View Article
humanWB

Clin Exp Immunol

Cultured lymphocytes' mitochondrial genome integrity is not altered by cladribine

Authors - Elina Järvinen
View Article