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  • Featured Product
  • KD/KO Validated

MRPS27 Polyclonal antibody

MRPS27 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17280-1-AP

Synonyms

KIAA0264, MRP S27, MRPS27, S27mt

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using MRPS27 Polyclonal antibody (17280-1-AP)

    WB analysis of A431 using 17280-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    WB analysis of human skeletal muscle using 17280-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    WB analysis of human heart using 17280-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using MRPS27 Polyclonal antibody (17280-1-AP)

    WB analysis of A431 using 17280-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17280-1-AP (MRPS27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using MRPS27 Polyclonal antibody (17280-1-AP)

    IP experiment of A431 using 17280-1-AP

    IP result of anti-MRPS27 (IP:17280-1-AP, 3ug; Detection:17280-1-AP 1:1000) with A431 cells lysate 1520ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human kidney using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human testis using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human testis using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human spleen using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human spleen using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human lung using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human lung using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPS27 Polyclonal antibody (17280-1-AP)

    IHC staining of human kidney using 17280-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17280-1-AP (MRPS27 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPS27 Polyclonal antibody (17280-1-AP)

    IF Staining of HepG2 using 17280-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPS27 rabbit poly (17280-1-AP, Green) and KRT18 mouse mAb (66187-1-Ig, Red) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L) for 17280-1-AP, and Alexa Fluor 594-conjugated Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

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Tested Applications

Positive WB detected inA431 cells, human heart tissue, human skeletal muscle tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman kidney tissue, human lung tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17280-1-AP targets MRPS27 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS27 fusion protein Ag11047 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S27
Calculated molecular weight 168 aa, 19 kDa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC030521
Gene symbol MRPS27
Gene ID (NCBI) 23107
RRIDAB_2180510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

Protocols

Product Specific Protocols
WB protocol for MRPS27 antibody 17280-1-APDownload protocol
IHC protocol for MRPS27 antibody 17280-1-APDownload protocol
IF protocol for MRPS27 antibody 17280-1-APDownload protocol
IP protocol for MRPS27 antibody 17280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.

Authors - Antonicka Hana H
View Article
humanWB

Nat Commun

RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.

Authors - Uwe Richter
View Article
mouseWB

J Clin Invest

AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.

Authors - Almajan Eva R ER
View Article
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
View Article
mouseWB

J Cell Biol

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Authors - Uwe Richter
View Article
humanWB

Proc Natl Acad Sci U S A

Mitochondrial ribosomal protein L12 selectively associates with human mitochondrial RNA polymerase to activate transcription.

Authors - Surovtseva Yulia V YV
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Xhuljana (Verified Customer) (03-01-2024)

WB in ripa lysate C2C12 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12 cells
MRPS27 Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:17280-1-AP)