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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS27 Monoclonal antibody, PBS Only

MRPS27 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1B3

Cat no : 66724-1-PBS

Synonyms

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using MRPS27 Monoclonal antibody (66724-1-Ig)

    WB analysis of LNCaP using 66724-1-Ig (same clone as 66724-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MRPS27 Monoclonal antibody (66724-1-Ig)

    WB analysis of HeLa using 66724-1-Ig (same clone as 66724-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using MRPS27 Monoclonal antibody (66724-1-Ig)

    WB analysis of A431 using 66724-1-Ig (same clone as 66724-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MRPS27 Monoclonal antibody (66724-1-Ig)

    WB analysis of HepG2 using 66724-1-Ig (same clone as 66724-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPS27 Monoclonal antibody (66724-1-Ig)

    IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPS27 Monoclonal antibody (66724-1-Ig)

    IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66724-1-PBS targets MRPS27 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MRPS27 fusion protein Ag11047 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S27
Calculated molecular weight 168 aa, 19 kDa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC030521
Gene symbol MRPS27
Gene ID (NCBI) 23107
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].