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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS28 Polyclonal antibody, PBS Only

MRPS28 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16378-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS28 Polyclonal antibody (16378-1-AP)

    WB analysis using 16378-1-AP (same clone as 16378-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16378-1-AP (MRPS28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MRPS28 Polyclonal antibody (16378-1-AP)

    IHC staining of human liver using 16378-1-AP (same clone as 16378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16378-1-AP (MRPS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16378-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using MRPS28 Polyclonal antibody (16378-1-AP)

    IHC staining of human liver using 16378-1-AP (same clone as 16378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16378-1-AP (MRPS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16378-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPS28 Polyclonal antibody (16378-1-AP)

    IF Staining of HepG2 using 16378-1-AP (same clone as 16378-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16378-1-AP (MRPS28 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16378-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPS28 Polyclonal antibody (16378-1-AP)

    IF Staining of HeLa using 16378-1-AP (same clone as 16378-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16378-1-AP (MRPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16378-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16378-1-PBS targets MRPS28 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS28 fusion protein Ag9354 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S28
Calculated molecular weight 187 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC010150
Gene symbol MRPS28
Gene ID (NCBI) 28957
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.