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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS34 Polyclonal antibody

MRPS34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15166-1-AP

Synonyms

Small ribosomal subunit protein mS34, S34mt, MRP-S34, MRPS12, MRP S34

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS34 Polyclonal antibody (15166-1-AP)

    WB analysis using 15166-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15166-1-AP (MRPS34 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MRPS34 Polyclonal antibody (15166-1-AP)

    IHC staining of human breast cancer using 15166-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15166-1-AP (MRPS34 antibody) at dilution of 1:500 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MRPS34 Polyclonal antibody (15166-1-AP)

    IF Staining of A549 using 15166-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MRPS34 antibody (15166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15166-1-AP targets MRPS34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS34 fusion protein Ag7287 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S34
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC001182
Gene symbol MRPS34
Gene ID (NCBI) 65993
RRIDAB_2144233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPS34 antibody 15166-1-APDownload protocol
IHC protocol for MRPS34 antibody 15166-1-APDownload protocol
IF protocol for MRPS34 antibody 15166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols