MRPS35 Polyclonal antibody

Name: MRPS35 Polyclonal antibody
Brand: Proteintech
SKU: 16457-1-AP

MRPS35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16457-1-AP

Synonyms

HDCMD11P, MDS023, MRP S28, MRP S35, MRPS28, MRPS35, PSEC0213, S28mt, S35mt

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 16457-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 16457-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16457-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16457-1-AP
IP result of anti-MRPS35 (IP:16457-1-AP, 4ug; Detection:16457-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of human colon cancer using 16457-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 16457-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 16457-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPS35 antibody (16457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16457-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human heart tissue, HepG2 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 29 publications below
IP	See 1 publications below

Product Information

16457-1-AP targets MRPS35 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MRPS35 fusion protein Ag9430 相同性解析による交差性が予測される生物種
Full Name	mitochondrial ribosomal protein S35
Calculated molecular weight	323 aa, 37 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC015862
Gene symbol	MRPS35
Gene ID (NCBI)	60488
RRID	AB_2146521
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.

Protocols

Product Specific Protocols
WB protocol for MRPS35 antibody 16457-1-AP	Download protocol
IHC protocol for MRPS35 antibody 16457-1-AP	Download protocol
IF protocol for MRPS35 antibody 16457-1-AP	Download protocol
IP protocol for MRPS35 antibody 16457-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals Authors - Timothy C Kenny View Article
mouse	WB	Sci Adv Adult-onset obesity is triggered by impaired mitochondrial gene expression. Authors - Kara L Perks View Article
mouse	WB	Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. Authors - Danielle L Rudler View Article
	WB	Cell Metab Tissue-Specific Loss of DARS2 Activates Stress Responses Independently of Respiratory Chain Deficiency in the Heart. Authors - Sukru Anil Dogan View Article
mouse	WB	J Clin Invest AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival. Authors - Almajan Eva R ER View Article
mouse	WB	Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. Authors - Tara R Richman View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS35 Polyclonal antibody

MRPS35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 16457-1-AP

WB analysis of human heart using 16457-1-AP

WB analysis of HeLa using 16457-1-AP

IP experiment of HeLa using 16457-1-AP

IHC staining of human colon cancer using 16457-1-AP

IHC staining of human colon cancer using 16457-1-AP

IF Staining of HeLa using 16457-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Tissue-Specific Loss of DARS2 Activates Stress Responses Independently of Respiratory Chain Deficiency in the Heart.

AFG3L2 supports mitochondrial protein synthesis and Purkinje cell survival.

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.