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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS36 Polyclonal antibody

MRPS36 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16279-1-AP

Synonyms

S36mt, MRP S36, KGD4, DC47, Alpha-ketoglutarate dehydrogenase subunit 4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPS36 Polyclonal antibody (16279-1-AP)

    IHC staining of human lung cancer using 16279-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16279-1-AP (MRPS36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MRPS36 Polyclonal antibody (16279-1-AP)

    IHC staining of human lung cancer using 16279-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16279-1-AP (MRPS36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MRPS36 Polyclonal antibody (16279-1-AP)

    IHC staining of human liver cancer using 16279-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16279-1-AP (MRPS36 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using MRPS36 Polyclonal antibody (16279-1-AP)

    IHC staining of human liver cancer using 16279-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16279-1-AP (MRPS36 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPS36 Polyclonal antibody (16279-1-AP)

    IF Staining of HeLa using 16279-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16279-1-AP (MRPS36 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16279-1-AP targets MRPS36 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS36 fusion protein Ag9359 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S36
Calculated molecular weight 103 aa, 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC015966
Gene symbol MRPS36
Gene ID (NCBI) 92259
RRIDAB_2144247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MRPS36 antibody 16279-1-APDownload protocol
IF protocol for MRPS36 antibody 16279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols