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  • Featured Product
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MRPS5 Polyclonal antibody

MRPS5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16428-1-AP

Synonyms

MRP S5, MRPS5, S5mt

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS5 Polyclonal antibody (16428-1-AP)

    WB analysis using 16428-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16428-1-AP (MRPS5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MRPS5 Polyclonal antibody (16428-1-AP)

    WB analysis of HeLa using 16428-1-AP

    WB result of MRPS5 antibody (16428-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS5 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPS5 Polyclonal antibody (16428-1-AP)

    IHC staining of human kidney using 16428-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16428-1-AP (MRPS5 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MRPS5 Polyclonal antibody (16428-1-AP)

    IHC staining of human kidney using 16428-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16428-1-AP (MRPS5 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 16428-1-AP is MRPS5 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS5 fusion protein Ag9672 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S5
Calculated molecular weight 430 aa, 48 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC014172
Gene symbol MRPS5
Gene ID (NCBI) 64969
RRIDAB_2878258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPS5 antibody 16428-1-APDownload protocol
IHC protocol for MRPS5 antibody 16428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Oncol

Porphyromonas gingivalis promotes malignancy and chemo-resistance via GSK3β-mediated mitochondrial oxidative phosphorylation in human esophageal squamous cell carcinoma

Authors - Yiwen Liu
View Article