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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRRF Monoclonal antibody (66942-1-Ig)

    WB analysis using 66942-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66942-1-Ig (MRRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRRF Monoclonal antibody (66942-1-Ig)

    IF Staining of HeLa using 66942-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRRF antibody (66942-1-Ig, Clone: 1G5C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

MRRF Monoclonal antibody

MRRF Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66942-1-Ig
Clone No.1G5C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RRF, Ribosome-releasing factor, mitochondrial, Ribosome-recycling factor, mitochondrial, mtRRF, MRFF

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, A431 cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66942-1-Ig targets MRRF in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MRRF fusion protein Ag28583 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosome recycling factor
Calculated molecular weight 262 aa, 29 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC013049
Gene Symbol MRRF
Gene ID (NCBI) 92399
RRIDAB_2882266
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96E11
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRRF antibody 66942-1-IgDownload protocol
IF protocol for MRRF antibody 66942-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols