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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MS4A12 Polyclonal antibody

MS4A12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13293-1-AP

Synonyms

Membrane-spanning 4-domains subfamily A member 12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MS4A12 Polyclonal antibody (13293-1-AP)

    WB analysis using 13293-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MS4A12 Polyclonal antibody (13293-1-AP)

    WB analysis using 13293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MS4A12 Polyclonal antibody (13293-1-AP)

    IHC staining of human colon cancer using 13293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MS4A12 Polyclonal antibody (13293-1-AP)

    IHC staining of human colon cancer using 13293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

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Tested Applications

Positive WB detected inSH-SY5Y cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13293-1-AP targets MS4A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MS4A12 fusion protein Ag3968 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 12
Calculated molecular weight 267 aa, 28 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC029793
Gene symbol MS4A12
Gene ID (NCBI) 54860
RRIDAB_10596915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MS4A12 antibody 13293-1-APDownload protocol
IHC protocol for MS4A12 antibody 13293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols