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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MS4A7 Polyclonal antibody (11864-1-AP)

    WB analysis using 11864-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11864-1-AP (MS4A7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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MS4A7 Polyclonal antibody

MS4A7 Polyclonal Antibody for WB, ELISA
Cat No. 11864-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

4SPAN2, CD20 antigen like 4, CD20L4, CFFM4, MS4A7, MS4A8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inBV-2 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11864-1-AP targets MS4A7 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MS4A7 fusion protein Ag2442 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 7
Calculated molecular weight 240 aa, 26 kDa
Observed molecular weight26 kDa
GenBank accession numberBC020673
Gene Symbol MS4A7
Gene ID (NCBI) 58475
RRIDAB_2144674
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human MS4A family currently includes 18 members, and these proteins have four putative transmembrane domains (tetraspan) and several protein kinase C (PKC) phosphorylation sites. Membrane-spanning 4-domains a7 (MS4A7) is a member of the MS4A family. It is expressed by myeloid cells. MS4A7 can act as a NAM-specific pathogenic factor that exacerbates metabolic dysfunction-associated steatohepatitis (MASH) progression in mice (PMID: 38478630). There are two isoforms of MS4A7 protein, with molecular weights of 26.14 kDa and 21.41 kDa respectively.

Protocols

Product Specific Protocols
WB protocol for MS4A7 antibody 11864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols