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KD/KO Validated

MSH2 Polyclonal antibody

Name: MSH2 Polyclonal antibody
Brand: Proteintech
SKU: 15520-1-AP

MSH2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15520-1-AP

Synonyms

MSH 2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15520-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15520-1-AP
WB results of MSH2 (15520-1-AP, 1:5000) antibody with si-Control and si-MSH2 transfected HeLa cells..
WB analysis using 15520-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15520-1-AP
IP result of anti-MSH2 (IP:15520-1-AP, 5ug; Detection:15520-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of human stomach cancer using 15520-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 15520-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15520-1-AP (MSH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsil using 15520-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human tonsil using 15520-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HeLa using 15520-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody (15520-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
FC experiment of HeLa using 15520-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MSH2 (15520-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 15520-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Caco-2 cells, SW480 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue, human tonsil tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 17 publications below
IHC	See 13 publications below
IF	See 3 publications below
IP	See 2 publications below

Product Information

15520-1-AP targets MSH2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MSH2 fusion protein Ag7835 相同性解析による交差性が予測される生物種
Full Name	mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight	105 kDa
Observed molecular weight	100 kDa-105 kDa
GenBank accession number	BC021566
Gene symbol	MSH2
Gene ID (NCBI)	4436
RRID	AB_2144799
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Protocols

Product Specific Protocols
WB protocol for MSH2 antibody 15520-1-AP	Download protocol
IHC protocol for MSH2 antibody 15520-1-AP	Download protocol
IF protocol for MSH2 antibody 15520-1-AP	Download protocol
IP protocol for MSH2 antibody 15520-1-AP	Download protocol
FC protocol for MSH2 antibody 15520-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Leukemia Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia Authors - Ji-Yuan Teng KO Validated View Article
human	WB	J Immunother Cancer KALRN mutations promote antitumor immunity and immunotherapy response in cancer. Authors - Mengyuan Li View Article
human	WB, IHC	Hum Mol Genet Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models. Authors - Dazhang Bai KD Validated View Article
human	WB	Cancer Prev Res (Phila) Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells. Authors - Jin Peng P View Article
human	IHC	Front Oncol Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence. Authors - Xuting Ran View Article
human	WB	J Biol Chem Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways. Authors - Mo Chen C View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MSH2 Polyclonal antibody

MSH2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 15520-1-AP

WB analysis of HeLa using 15520-1-AP

WB analysis using 15520-1-AP

IP experiment of HeLa using 15520-1-AP

IHC staining of human stomach cancer using 15520-1-AP

IHC staining of human stomach cancer using 15520-1-AP

IHC staining of human tonsil using 15520-1-AP

IHC staining of human tonsil using 15520-1-AP

IF Staining of HeLa using 15520-1-AP

FC experiment of HeLa using 15520-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia

KALRN mutations promote antitumor immunity and immunotherapy response in cancer.

Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.

Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.

Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.

Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways.