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  • Featured Product
  • KD/KO Validated

MSH2 Monoclonal antibody, PBS Only (Capture)

MSH2 Monoclonal Antibody for WB, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

2H5F5

Cat no : 60161-1-PBS

Synonyms

MSH 2, 2H5F5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MSH2 Monoclonal antibody (60161-1-Ig)

    WB analysis of HeLa using 60161-1-Ig (same clone as 60161-1-PBS)

    WB results of MSH2 (60161-1-Ig, 1:1000) antibody with si-Control and si-MSH2 transfected HeLa cells.. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MSH2 Monoclonal antibody (60161-1-Ig)

    WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MSH2 Monoclonal antibody (60161-1-Ig)

    WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH2 Monoclonal antibody (60161-1-Ig)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH2 Monoclonal antibody (60161-1-Ig)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH2 Monoclonal antibody (60161-1-Ig)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH2 Monoclonal antibody (60161-1-Ig)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH2 Monoclonal antibody (60161-1-Ig)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH2 Monoclonal antibody (60161-1-Ig)

    IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1, MSH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60161-1-PBS. Detection antibody: 60161-2-PBS. Standard:Ag7835. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60161-1-PBS targets MSH2 as part of a matched antibody pair:

MP50235-1: 60161-1-PBS capture and 60161-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MSH2 fusion protein Ag7835 相同性解析による交差性が予測される生物種
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC021566
Gene symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.