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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSH2 Recombinant antibody (82775-1-RR)

    WB analysis using 82775-1-RR (same clone as 82775-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82775-1-RR (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using MSH2 Recombinant antibody (82775-1-RR)

    WB analysis of HeLa using 82775-1-RR (same clone as 82775-1-PBS)

    WB result of MSH2 antibody (82775-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH2 transfected HeLa cells. This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human colon cancer using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82775-1-RR (MSH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human tonsillitis using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using MSH2 Recombinant antibody (82775-1-RR)

    IHC staining of human colon using 82775-1-RR (same clone as 82775-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82775-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82775-1-RR

    Affinity and Kinetic Characterization of 82775-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82775-1-RR against Human MSH2 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

MSH2 Recombinant antibody, PBS Only

MSH2 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82775-1-PBS
Clone No.6O4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

MSH 2, 6O4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82775-1-PBS targets MSH2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MSH2 fusion protein Ag7835 相同性解析による交差性が予測される生物種
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight 100 kDa-105 kDa
GenBank accession numberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43246
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.