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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MSH3 Polyclonal antibody, PBS Only

MSH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22393-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSH3 Polyclonal antibody (22393-1-AP)

    WB analysis using 22393-1-AP (same clone as 22393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22393-1-AP (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using MSH3 Polyclonal antibody (22393-1-AP)

    IP experiment of HeLa using 22393-1-AP (same clone as 22393-1-PBS)

    IP result of anti-MSH3 (IP:22393-1-AP, 4ug; Detection:22393-1-AP 1:1000) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH3 Polyclonal antibody (22393-1-AP)

    IHC staining of human colon cancer using 22393-1-AP (same clone as 22393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH3 Polyclonal antibody (22393-1-AP)

    IHC staining of human colon cancer using 22393-1-AP (same clone as 22393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using MSH3 Polyclonal antibody (22393-1-AP)

    IF Staining of HT-29 using 22393-1-AP (same clone as 22393-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using MSH3 antibody (22393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 22393-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22393-1-PBS targets MSH3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSH3 fusion protein Ag18060 相同性解析による交差性が予測される生物種
Full Name mutS homolog 3 (E. coli)
Calculated molecular weight 1137 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC130434
Gene symbol MSH3
Gene ID (NCBI) 4437
RRIDAB_11232405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).