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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MSH3 Recombinant antibody (82845-3-RR)

    WB analysis using 82845-3-RR (same clone as 82845-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MSH3 Recombinant antibody, PBS Only (82845-3-PBS)

    IF Staining of HeLa using 82845-3-PBS

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH3 antibody (82845-3-PBS, Clone: 1J23 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U2OS using 82845-3-RR (same clone as 82845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using MSH3 Recombinant antibody (82845-3-RR)

    IF Staining of U-251 using 82845-3-RR (same clone as 82845-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82845-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82845-3-RR

    Affinity and Kinetic Characterization of 82845-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

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MSH3 Recombinant antibody, PBS Only

MSH3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82845-3-PBS
Clone No.1J23

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Mismatch repair protein 1, hMSH3, DUG, DUC1, Divergent upstream protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82845-3-PBS targets MSH3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MSH3 fusion protein Ag18060 相同性解析による交差性が予測される生物種
Full Name mutS homolog 3 (E. coli)
Calculated molecular weight 1137 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC130434
Gene Symbol MSH3
Gene ID (NCBI) 4437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20585
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

Protocols

Product Specific Protocols
IF protocol for MSH3 antibody 82845-3-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols