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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MSH6 Monoclonal antibody (66172-1-Ig)

    WB analysis of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    WB result of MSH6 antibody (66172-1-Ig, 1:1000) with si-Control and si-MSH6 transfected HEK293 cells. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using MSH6 Monoclonal antibody (66172-1-Ig)

    WB analysis of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using MSH6 Monoclonal antibody (66172-1-Ig)

    WB analysis of U2OS using 66172-1-Ig (same clone as 66172-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MSH6 Monoclonal antibody (66172-1-Ig)

    WB analysis using 66172-1-Ig (same clone as 66172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using MSH6 Monoclonal antibody (66172-1-Ig)

    WB analysis of HepG2 using 66172-1-Ig (same clone as 66172-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MSH6 Monoclonal antibody (66172-1-Ig)

    IP experiment of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    IP result of anti-MSH6 (IP:66172-1-Ig, 4ug; Detection:66172-1-Ig 1:600) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-1-Ig)

    IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Ramos cells using MSH6 Monoclonal antibody (66172-1-Ig)

    IHC staining of Ramos using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-1-Ig)

    IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-1-Ig)

    IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-1-Ig)

    IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-1-Ig)

    IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-1-Ig)

    IHC staining of human colon cancer using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-1-Ig (MSH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MSH6 Monoclonal antibody (66172-1-Ig)

    IF Staining of U2OS using 66172-1-Ig (same clone as 66172-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH6 antibody (66172-1-Ig, Clone: 2E10B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using MSH6 Monoclonal antibody (66172-1-Ig)

    FC experiment of HEK-293 using 66172-1-Ig (same clone as 66172-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug MSH6 Monoclonal antibody (66172-1-Ig, Clone:2E10B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66172-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

MSH6 Monoclonal antibody, PBS Only

MSH6 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66172-1-PBS
Clone No.2E10B2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

hMSH6, GTMBP, GTBP, G/T mismatch-binding protein, G/T mismatch binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66172-1-PBS targets MSH6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MSH6 fusion protein Ag12645 相同性解析による交差性が予測される生物種
Full Name mutS homolog 6 (E. coli)
Calculated molecular weight 153 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC004246
Gene Symbol MSH6
Gene ID (NCBI) 2956
RRIDAB_2881567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52701
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.