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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colon cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colon using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human appendicitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human rectal cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human colon cancer using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MSH6 Monoclonal antibody (66172-2-Ig)

    IHC staining of human tonsillitis using 66172-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66172-2-Ig (MSH6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for IHC, ELISA
Cat No. 66172-2-Ig
Clone No.5B5A7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Synonyms

hMSH6, GTMBP, GTBP, G/T mismatch-binding protein, G/T mismatch binding protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human colon tissue, human colon cancer tissue, human rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66172-2-Ig targets MSH6 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MSH6 fusion protein Ag12645 相同性解析による交差性が予測される生物種
Full Name mutS homolog 6 (E. coli)
Calculated molecular weight 153 kDa
Observed molecular weight150-160 kDa
GenBank accession numberBC004246
Gene Symbol MSH6
Gene ID (NCBI) 2956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52701
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Protocols

Product Specific Protocols
IHC protocol for MSH6 antibody 66172-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols