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Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using MSMP Polyclonal antibody (19526-1-AP)

    WB analysis of DU 145 using 19526-1-AP

    WB result of MSMP antibody (19526-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSMP transfected DU 145 cells.

  • Western Blot (WB) analysis of various lysates using MSMP Polyclonal antibody (19526-1-AP)

    WB analysis using 19526-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MSMP Polyclonal antibody (19526-1-AP)

    WB analysis using 19526-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MSMP Polyclonal antibody (19526-1-AP)

    IF Staining of PC-3 using 19526-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MSMP antibody (19526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

MSMP Polyclonal antibody

MSMP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19526-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MSMP, PC 3, PC3 secreted microprotein, PSMP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, DU 145 cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19526-1-AP targets MSMP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name microseminoprotein, prostate associated
Calculated molecular weight 15 kDa
Observed molecular weight12 kDa, 20 kDa
GenBank accession numberNM_001044264
Gene Symbol MSMP
Gene ID (NCBI) 692094
RRIDAB_3085579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1L6U9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSMP, also named PSMP, belongs to the beta-microseminoprotein family. MSMP is highly conserved, sharing 91 to 94% amino acid identity with its rodent and dog homologs. RT-PCR of 27 human tissues detected PSMP expression only in trachea and, to a lesser extent, testis. MSMP was highly expressed in PC3 cells, but not in 2 other prostate cancer cell lines. Western blot analysis revealed 20 kDa and 12 kDa bands PSMP maybe due to posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for MSMP antibody 19526-1-APDownload protocol
IF protocol for MSMP antibody 19526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols